Biosynthetic and Genetic Relationships

نویسنده

  • Ronald D. Plattner
چکیده

Fumonisins are mycotoxins produced by the maize pathogen Gibberel/a fujikuroi mating population A and frequently contaminate maize. Wild-type G. fujikuroi produces four B-series fumonisins, FB" FBz, FB3 and FB4 . These toxins are identical in structure except for the number and positions of hydroxyls along their linear carbon backbone. To elucidate the genetic and biosynthetic relationships among these fumonisins, we conducted meiotic and biochemical analyses of G. fujikuroi mutants with altered fumonisin production that resulted from defective alleles at three loci, Fum 1, Fum2 and Fum3. These mutants produced either no fumonisins, only FBz and FB4 , or only FB3 and FB4 . Genetic analyses revealed the orientation of the Fum loci along linkage group 1 of the fungus. The mutants were grown together in pair-wise combinations to determine if their fumonisin production phenotypes could be complemented. When FBT and FBrproducing mutants were grown together, complementation occurred. However, when a nonproducing mutant was grown with a FBr or FB3-producing mutant, complementation did not occur or was incomplete. When purified FBz, FB3 , or FB4 was fed to mutant cultures, FB4 was converted primarily to FBz, FB3 was converted to FB, and FBz was not converted. The results from these assays suggest a previously unrecognized branch in the fumonisin biosynthetic pathway. Published in 1999 by John Wiley & Sons, Ltd.

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تاریخ انتشار 2007