Biosynthetic and Genetic Relationships
نویسنده
چکیده
Fumonisins are mycotoxins produced by the maize pathogen Gibberel/a fujikuroi mating population A and frequently contaminate maize. Wild-type G. fujikuroi produces four B-series fumonisins, FB" FBz, FB3 and FB4 . These toxins are identical in structure except for the number and positions of hydroxyls along their linear carbon backbone. To elucidate the genetic and biosynthetic relationships among these fumonisins, we conducted meiotic and biochemical analyses of G. fujikuroi mutants with altered fumonisin production that resulted from defective alleles at three loci, Fum 1, Fum2 and Fum3. These mutants produced either no fumonisins, only FBz and FB4 , or only FB3 and FB4 . Genetic analyses revealed the orientation of the Fum loci along linkage group 1 of the fungus. The mutants were grown together in pair-wise combinations to determine if their fumonisin production phenotypes could be complemented. When FBT and FBrproducing mutants were grown together, complementation occurred. However, when a nonproducing mutant was grown with a FBr or FB3-producing mutant, complementation did not occur or was incomplete. When purified FBz, FB3 , or FB4 was fed to mutant cultures, FB4 was converted primarily to FBz, FB3 was converted to FB, and FBz was not converted. The results from these assays suggest a previously unrecognized branch in the fumonisin biosynthetic pathway. Published in 1999 by John Wiley & Sons, Ltd.
منابع مشابه
Activation of Lignin Biosynthetic Enzymes During Internodal Development of Aeluropus littoralis Exposed to NaCl
Lignin is one of the major characteristics of plant secondary cell wall that provides structural rigidity for the cells and tissues and hydrophobicity to tracheary elements. Internode tissues of Aeluropus littoralis as a halophyte grass were sampled at different developmental stages (from the first to the fifth internodes ) and under different NaCl concentrations. The influences of NaCl and int...
متن کاملComparison of acute toxicity (LC50) of chemical and biosynthetic by Sargasum angustifolium nanosilvers in Asian sea bass (Lates calcarifer)
This study was aimed to biosynthesis of silver nanoparticles using the seaweeds, Sargassum angustifolium and Comparison of acute toxicity (LC50) of commercial silver nanoparticles and biosynthetic nanoparticles performed by seaweed Sargasum angustifolium in Asian sea bass (Lates calcarifer) in 1397 in Ahvaz.After preparing extracts from Sargassum and biosynthesize the silver nanoparticles by ad...
متن کاملGenetic relationships of Iranian coastline ship sturgeon (Acipenser nudiventris) samples and Ural population based on microsatellite DNA
متن کامل
The Application of Recursive Mixed Models for Estimating Genetic and Phenotypic Relationships between Calving Difficulty and Lactation Curve Traits in Iranian Holsteins: A Comparison with Standard Mixed Models
In the present study, records on 22872 first-parity Holsteins collected from 131 herds by the Animal Breeding and Improvement Center of Iran from 1995 to 2014 were considered to estimate genetic and phenotypic relationships between calving difficulty (CD) and the lactation curve traits, including initial milk yield (Ap), ascending (Bp) and descending (Cp) slope of the lactation curves, peak mil...
متن کاملGenetic Relationships among Three Yarrow Species Based on Phenotypic Traits and Peroxidase Profiling
Fifteen yarrow populations from different species Achillea millefolium L., A. biebersteinii L. and A. nobilis, from different geographical areas of Iran were studied using 24 morphological traits and peroxidase profiles. Comparison of mean values of different phenotypic traits show A. millefolium and A. biebersteinii L. had higher plant height and crown diameter; however, A. nobilis had higher ...
متن کاملDoes Biosynthetic Silver Nanoparticles Are More Stable With Lower Toxicity than Their Synthetic Counterparts?
Control of size and shape is a challenge in nanoparticle synthesis. Synthetic and biosynthetic (both extracellular and intracellular) methods are used to prepare silver nanoparticle (SNP). In this study, the behavior of three strains of Staphylococcus aureus (S. aureus) was investigated in the presence of silver nitrate intra- and extracellularly. S. aureus strains biosynthesized SNPs intracell...
متن کامل